Figure 2
From: Radiosensitization of colorectal carcinoma cell lines by histone deacetylase inhibition
Cell cycle regulatory factors following exposure to ionizing radiation (IR). Four colorectal carcinoma cell lines (HCT116, Co115, SW620, and KM20L2) were exposed (+) to 8 Gy of IR, or left unexposed (-), and further incubated for the indicated time periods before analysis. Upper panel: Protein expression levels of Plk1, p53, Cyclin D1, and p21 were analyzed by Western blot immunostaining, using α-tubulin as protein loading control. Lower panel: mRNA expression levels of PLK, CCND1, and CDKN1A were analyzed by Northern blot hybridization, using 18S rRNA as RNA loading control.