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Table 1 Primary antibodies used for immunohistochemical staining

From: Dynamics in the expression of programmed death ligand 1 and cluster of differentiation 163 in the tumor microenvironment of uterine cervical cancer: a single-center retrospective study

Clone

Antigen

Dilution

Manufacturer

Cell expression site in the TME

KP-1

CD68

1:1200

DakoCytomation, Glostrup, Denmark

Cell membrane

10D6

CD163

1:100

Leica Microsystems, Newcastle, UK

Cell membrane

EPR4877 (2)

PD-1

1:200

Abcam, Cambridge, MA, USA

Cell membrane

E1L3N

PD-L1

1:100

Cell Signaling Technology, Inc., Danvers, MA, USA

Cell membrane

4B11

CD8

1:200

Leica Microsystems, Newcastle, UK

Cell membrane

236A/E7

FoxP3

1:100

Abcam, Cambridge, MA, USA

Nucleus

54/HIF-1α

HIF-1α

1:50

BD Biosciences, New Jersey, USA

Nucleus

  1. TME tumor microenvironment, CD cluster of differentiation, PD-1 programmed death 1, PD-L1 programmed death ligand 1, FoxP3 forkhead boxprotein P3, HIF-1α hypoxia-inducible factor-1α