Clone | Antigen | Dilution | Manufacturer | Cell expression site in the TME |
---|---|---|---|---|
KP-1 | CD68 | 1:1200 | DakoCytomation, Glostrup, Denmark | Cell membrane |
10D6 | CD163 | 1:100 | Leica Microsystems, Newcastle, UK | Cell membrane |
EPR4877 (2) | PD-1 | 1:200 | Abcam, Cambridge, MA, USA | Cell membrane |
E1L3N | PD-L1 | 1:100 | Cell Signaling Technology, Inc., Danvers, MA, USA | Cell membrane |
4B11 | CD8 | 1:200 | Leica Microsystems, Newcastle, UK | Cell membrane |
236A/E7 | FoxP3 | 1:100 | Abcam, Cambridge, MA, USA | Nucleus |
54/HIF-1α | HIF-1α | 1:50 | BD Biosciences, New Jersey, USA | Nucleus |