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Fig. 5 | Radiation Oncology

Fig. 5

From: ΔNp63α transcriptionally represses p53 target genes involved in the radiation-induced DNA damage response

Fig. 5

Analyses of iPS-DNs and iPS-KCs expressing ΔNp63α ectopically and entopically, respectively. (a) Immunostaining images of ΔNp63α in iPS-DNs ectopically expressing ΔNp63α with the doxorubicin (Dox) Tet-off system. Scale bar, 20 μm. (b) DDR marker mRNA expression in iPS-DNs post-irradiation. All values were scaled to the expression level of GAPDH as an internal control. Data represent the means and SEs of three independent assays. (c) The frequencies of cell cycle phase in iPS-DN with or without ΔNp63α expression at 24 h after X-irradiation, which were detected by FCM. (d) CC3-positive apoptotic cells detected by FCM in iPS-DNs post-irradiation. (e) Western blotting analysis for iPS-DNs post-irradiation. (f) ChIP analysis of BAX and CDKN1A promoters by anti-p53 antibody in iPS-DNs. Data represent the means of triplicate experiments. Nega: negative control, pro: promoter. (g) Immunostaining images for iPS-KCs entopically expressing ΔNp63α. Scale bar, 20 μm. (h) mRNA expression ratio of Bax and CDKN1A in iPS-KCs post-irradiation (*P < 0.05 vs. 0 Gy by Mann–Whitney U test). (i) Measurement of EdU-positivity frequency in iPS-KCs. (j) Apoptotic cell frequencies in hiPSCs and iPS-KCs at 24 h after irradiation. Data in all figure panels except (h) were analysed with Student’s t test (*P < 0.05, **P < 0.01)

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