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Fig. 8 | Radiation Oncology

Fig. 8

From: Ganetespib selectively sensitizes cancer cells for proximal and distal spread-out Bragg peak proton irradiation

Fig. 8

Rad51 immunoblot of A549 cells—8 h and 24 h after irradiation. Immunoblotting of Rad51 protein levels in response to photon irradiation and proton irradiation at a proximal or distal SOBP positions. A549 cells were harvested A 8 h and B 24 h subsequent to 4 Gy of the indicated type of irradiation. Ganetespib or sham treatment was administered 1 h prior to irradiation and replaced by fresh drug-containing medium after the irradiation. A control lysate was utilized for normalizing protein levels in between the membranes/time point (dotted line) with error bars indicating the standard deviation. Data from 3 independent biological replicates was compiled but differences did not reach significance as assessed by unpaired, two-tailed t tests with Welch’s Correction due to the higher variability in immunoblotting results and the limited sample number

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