Fig. 7

Cell cycle analysis of A549 in response to ganetespib and different radiation modalities. A Neither pretreatment nor long term incubation with low-dosed ganetespib altered the fraction of cells in S/G2/M phase (filled red). Cells fixated B 8 h, C 24 h, and D 48 h after receiving 4 Gy of the respective irradiation exhibit an accumulation in S/G2/M phase within 8 h followed by a decline at the 24 h and 48 h time point. A more pronounced fraction of cells in S/G2/M phase could be observed in proton-irradiated cells at the 8 h, 24 h, and 48 h time point which was significant at 8 h for both proton modalities and at 24 h for the distal position. 10 nM ganetespib consistently suppressed the fraction of irradiated cells in S/G2/M phase. Single cells were gated by FSC-H/FSC-A and G1, S and early G2, and late G2 and M phase was gated by DNA content and BrdU uptake. A biological duplicate could be obtained and statistical testing was performed by unpaired, two-tailed t test with Welch’s Correction with error bars indicating the standard deviation