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Fig. 1 | Radiation Oncology

Fig. 1

From: Development of a model for fibroblast-led collective migration from breast cancer cell spheroids to study radiation effects on invasiveness

Fig. 1

Fibroblast-led invasion of breast cancer cells into Spheroid Invasion Matrix. a Monocultured BJ1-hTert and HDF fibroblast spheroids and coculture spheroids with mammary carcinoma cells MCF-7 and BT474, respectively, were embedded in matrix one day after seeding. Bright field images were taken on day 7 after embedding. Size bar is 500 µm. b Cancer cells stained red by expression of tagRFP (MCF-7) or by live-cell staining (BT474) were seeded with green fibroblasts obtained by live-cell staining in ULA plates. Spheroids were embedded in matrix one day after seeding and confocal images were taken on day 5 after embedding. Overviews and close-ups of fibroblast-led collective cancer cell migration are shown for all coculture combinations. White size bar is 500 µm and yellow bar is 200 µm

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