Fig. 5

Representative axial histological images of carbon ion and photon irradiated animals with paresis II and their respective age-matched controls. a–d Luxol fast blue staining revealing focal demyelination and necrosis (asterisks) in the S. alba of irradiated animals while control animals showed dense tissue structure (scale bar 200 µm). e–h Endogenous albumin extravasation, represented by brown precipitation, indicates the breakdown of the BSCB. i–l Irradiated segments show a severe decrease in intact blood vessels (CD34, red) and breakdown of the BSCB (EBA, green). Overlap of CD34 and EBA appears yellow. Cell nuclei were counter stained with DAPI (scale bar 50 µm)