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Fig. 4 | Radiation Oncology

Fig. 4

From: The clonogenic assay: robustness of plating efficiency-based analysis is strongly compromised by cellular cooperation

Fig. 4

Data taken from a

Cellular cooperation is a very common phenomenon observed in various cell lines across different cancer entities in vitro. a Geometric series of twelve different cell densities were seeded for various cancer cell lines of the pancreas (blue), lung (turquoise), brain (orange), head and neck (violet), and breast (dark red) and combined with the data from Fig. 1 (Three to five independent biological replicates per cell line). For each cell line, a pair of b-values (0 Gy vs. 4 Gy) is shown in the graph of the respective tumor entity. Areas highlighted in pale brown indicate cooperative growth behavior resulting in less than borderline robustness if analyzed by PE-based approaches. Error bars represent standard errors of fitted b-values, and boxplots for entity-specific distributions of b-values are shown at the edges of the graphs. b Bar diagrams indicating the percentage of cell lines displaying a relevant degree of cellular cooperation in vitro.

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