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Table 2 amount of DNA assessed, coding and amino acid changes, sequencing depths and VAFs for each sample

From: Early circulating tumour DNA kinetics measured by ultra-deep next-generation sequencing during radical radiotherapy for non-small cell lung cancer: a feasibility study

Sample ID Isolated DNA Mass (ng) Gene Coding Change Amino Acid Change Allele Fraction No. Mutant Molecules per mL Unique depth
Patient 1-day 1 22.49 IDH2 c.419G > A p.Arg140Gln 0.17% 2.6 4506
Patient 1-day 3 23.21 IDH2 c.419G > A p.Arg140Gln 0.05% 0.783 4622
Patient 2-day 1 38.61 TP53 c.772G > T p.Glu258* 0.06% 1.67 6361
Patient 2-day 3 35.69 Variant ND N/A N/A N/A N/A 5625
Patient 2-day 7 37.83 Variant ND N/A N/A N/A N/A 5334
Patient 3-day 1 30.16 EGFR c.2573 T > G p.Leu858Arg 3.35% 66.7 4579
Patient 3-day 3 39.65 EGFR c.2573 T > G p.Leu858Arg 2.82% 73.7 4911
Patient 3-day 7 40.37 EGFR c.2573 T > G p.Leu858Arg 1.75% 51.9 6116
Patient 4-day 1 26.33 TP53 c.473G > C p.Arg158Pro 0.40% 6.98 5630
Patient 4-day 1 26.33 SMAD4 c.931C > T p.Gln311* 0.68% 11.8 5630
Patient 4-day 1 26.33 BRAF c.1780G > A p.Asp594Asn 1.13% 19.7 5630
Patient 4-day 3 43.42 TP53 c.473G > C p.Arg158Pro 1.00% 28.7 6782
Patient 4-day 3 43.42 TP53 c.434 T > C p.Leu145Pro 0.11% 3.16 6782
Patient 4-day 3 43.42 SMAD4 c.931C > T p.Gln311* 0.60% 17.2 6782
Patient 4-day 3 43.42 BRAF c.1780G > A p.Asp594Asn 1.85% 53.2 6782
Patient 4-day 7 32.89 TP53 c.473G > C p.Arg158Pro 0.68% 17.1 5662
Patient 4-day 7 32.89 SMAD4 c.931C > T p.Gln311* 0.56% 14.1 5662
Patient 4-day 7 32.89 BRAF c.1780G > A p.Asp594Asn 1.61% 40.7 5662
Patient 5-day 1 17.94 TP53 c.743G > A p.Arg248Gln 1.12% 13.3 3806
Patient 5-day 1 17.94 TP53 c.476C > T p.Ala159Val 0.26% 3.05 3806
Patient 5-day 3 13.13 TP53 c.743G > A p.Arg248Gln 1.56% 13.5 2430
Patient 5-day 7 33.09 TP53 c.743G > A p.Arg248Gln 1.36% 29.7 5143
Patient 5-day 7 33.09 TP53 c.476C > T p.Ala159Val 0.22% 4.81 5143
  1. (*ND Not detected, N/A Not applicable)