Table 2 amount of DNA assessed, coding and amino acid changes, sequencing depths and VAFs for each sample
Sample ID | Isolated DNA Mass (ng) | Gene | Coding Change | Amino Acid Change | Allele Fraction | No. Mutant Molecules per mL | Unique depth |
|---|---|---|---|---|---|---|---|
Patient 1-day 1 | 22.49 | IDH2 | c.419G > A | p.Arg140Gln | 0.17% | 2.6 | 4506 |
Patient 1-day 3 | 23.21 | IDH2 | c.419G > A | p.Arg140Gln | 0.05% | 0.783 | 4622 |
Patient 2-day 1 | 38.61 | TP53 | c.772G > T | p.Glu258* | 0.06% | 1.67 | 6361 |
Patient 2-day 3 | 35.69 | Variant ND | N/A | N/A | N/A | N/A | 5625 |
Patient 2-day 7 | 37.83 | Variant ND | N/A | N/A | N/A | N/A | 5334 |
Patient 3-day 1 | 30.16 | EGFR | c.2573 T > G | p.Leu858Arg | 3.35% | 66.7 | 4579 |
Patient 3-day 3 | 39.65 | EGFR | c.2573 T > G | p.Leu858Arg | 2.82% | 73.7 | 4911 |
Patient 3-day 7 | 40.37 | EGFR | c.2573 T > G | p.Leu858Arg | 1.75% | 51.9 | 6116 |
Patient 4-day 1 | 26.33 | TP53 | c.473G > C | p.Arg158Pro | 0.40% | 6.98 | 5630 |
Patient 4-day 1 | 26.33 | SMAD4 | c.931C > T | p.Gln311* | 0.68% | 11.8 | 5630 |
Patient 4-day 1 | 26.33 | BRAF | c.1780G > A | p.Asp594Asn | 1.13% | 19.7 | 5630 |
Patient 4-day 3 | 43.42 | TP53 | c.473G > C | p.Arg158Pro | 1.00% | 28.7 | 6782 |
Patient 4-day 3 | 43.42 | TP53 | c.434 T > C | p.Leu145Pro | 0.11% | 3.16 | 6782 |
Patient 4-day 3 | 43.42 | SMAD4 | c.931C > T | p.Gln311* | 0.60% | 17.2 | 6782 |
Patient 4-day 3 | 43.42 | BRAF | c.1780G > A | p.Asp594Asn | 1.85% | 53.2 | 6782 |
Patient 4-day 7 | 32.89 | TP53 | c.473G > C | p.Arg158Pro | 0.68% | 17.1 | 5662 |
Patient 4-day 7 | 32.89 | SMAD4 | c.931C > T | p.Gln311* | 0.56% | 14.1 | 5662 |
Patient 4-day 7 | 32.89 | BRAF | c.1780G > A | p.Asp594Asn | 1.61% | 40.7 | 5662 |
Patient 5-day 1 | 17.94 | TP53 | c.743G > A | p.Arg248Gln | 1.12% | 13.3 | 3806 |
Patient 5-day 1 | 17.94 | TP53 | c.476C > T | p.Ala159Val | 0.26% | 3.05 | 3806 |
Patient 5-day 3 | 13.13 | TP53 | c.743G > A | p.Arg248Gln | 1.56% | 13.5 | 2430 |
Patient 5-day 7 | 33.09 | TP53 | c.743G > A | p.Arg248Gln | 1.36% | 29.7 | 5143 |
Patient 5-day 7 | 33.09 | TP53 | c.476C > T | p.Ala159Val | 0.22% | 4.81 | 5143 |