Fig. 6

PAF1 promotes Pol II promoter-proximal pausing and causes decreased transcription of IER5 by regulating the enhancers. a&b Results of qRT-PCR for IER5 mRNA showing that knockout of the enhancers caused PAF1 knockdown or IR to no longer promote IER5 transcription in either Siha (a) nor Hela (b) cells. c&d ChIP-qPCR analysis of Pol II occupancy at the promoter and gene body of IER5 revealing that both PAF1 knockdown and IR increased the PRR value, while after knocking out the enhancers, PAF1 knockdown or IR had almost no effect on PRR in Siha (c) and Hela (d). N = 3 in each group. All data are expressed as the mean (S.D.): * P < 0.05; ** P < 0.01; and *** P < 0.001. si-PAF1, PAF1 knockdown; NONT, negative control for PAF1; EN_KO, enhancer1/2 knockout; WT, wild type as negative control for EN_KO; IR, irradiation treatment; NT, non-treatment; PRR, Pol II release ratio