Fig. 3

Knocking down PAF1 enhances the efficacy of the radiotherapy of CC cells. a CCK-8 assay of Siha and Hela cells treated with radiation showing significantly reduced proliferation rates (Siha cells: P = 0.002, Hela cells: P = 0.019), and knocking down the PAF1 gene enhanced the cell-killing ability upon cell IR (Siha cells: P = 0.004, Hela cells: P = 0.018). b FCM-cell cycle analysis (24 h after IR) with propidium iodide (PI) staining shows PAF1 knockdown and IR resulted in an increased percentage of both Siha and Hela cells blocked in the G2/M cell cycle. c FCM-apoptosis analysis (72 h after cell IR) with PI and annexin V-FITC staining shows an increased percentage of apoptotic PAF1-knockdown Siha and Hela cells (Siha cells: 33.6%, Hela cells: 35.3%) subjected to IR (Siha cells: 3.5 Gy, Hela cells: 4 Gy) compared with that of the control cells (Siha cells: 27.0%, Hela cells: 21.2%), N = 3 in each group. All data are expressed as the mean (S.D.): * P < 0.05 and ** P < 0.01