Fig. 1From: Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcriptionExpression of PAF1 and IER5 in cervical cancer samples. PAF1 and IER5 levels were detected by Western blotting: (a) Representative immunoblots showing PAF1 overexpression in CC sample cells and very low expression of IER5 in both cervical cancer and normal cervix; (b) Densitometric quantification of PAF1 and IER5 in the CC and NC groups, as evaluated by Western blotting. PAF1 and IER5 levels were also detected by IHC: Representative images of PAF1 (c) and IER5 (d) proteins staining of cervical cancer and normal cervical tissues by IHC, taken at 100× (scale bar = 100 μm) and 400× (scale bar = 50 μm) magnification; (e) Relative MOD quantification of PAF1 and IER5 in the CC and NC groups. f Quantitative RT-PCR analysis showed increased expression of PAF1 in the CC sample cells, and low expression of IER5 in both CC and NC groups, normalized to that of β-actin. All data are expressed as the mean (S.D.): * P < 0.05, **P < 0.01, *** P < 0.001. NC, normal cervix; CC, cervical cancerBack to article page