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Fig. 1 | Radiation Oncology

Fig. 1

From: Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription

Fig. 1

Expression of PAF1 and IER5 in cervical cancer samples. PAF1 and IER5 levels were detected by Western blotting: (a) Representative immunoblots showing PAF1 overexpression in CC sample cells and very low expression of IER5 in both cervical cancer and normal cervix; (b) Densitometric quantification of PAF1 and IER5 in the CC and NC groups, as evaluated by Western blotting. PAF1 and IER5 levels were also detected by IHC: Representative images of PAF1 (c) and IER5 (d) proteins staining of cervical cancer and normal cervical tissues by IHC, taken at 100× (scale bar = 100 μm) and 400× (scale bar = 50 μm) magnification; (e) Relative MOD quantification of PAF1 and IER5 in the CC and NC groups. f Quantitative RT-PCR analysis showed increased expression of PAF1 in the CC sample cells, and low expression of IER5 in both CC and NC groups, normalized to that of β-actin. All data are expressed as the mean (S.D.): * P < 0.05, **P < 0.01, *** P < 0.001. NC, normal cervix; CC, cervical cancer

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