Fig. 3

NOX4 mRNA and protein levels are increased in PBMCs after ex-vivo irradiation. a mRNA expression of NOX family members in all patients (n = 20; n = 7 with and n = 13 without grade ≥ 2 bf+) in non-irradiated PBMC samples was analyzed by qRT-PCR. ND, not detected (below threshold). b NOX4 mRNA expression is increased in all patients (n = 20) at 24 h after irradiation (8 Gy) compared with non-irradiated samples (0 Gy) (Wilcoxon matched-pairs signed rank test). c NOX4 protein expression in all patients (n = 20) at 24 h after irradiation (8 Gy) compared with non-irradiated samples (0 Gy) (Wilcoxon matched-pairs signed rank test). d Western blot analysis of NOX4 expression in all 20 patients before (−) and at 24 h after 8 Gy-irradiation (+). β-actin was used as loading control. Data are the mean ± SEM; *p < 0.05 (paired t-test)