Fig. 4
From: Development of a high-throughput γ-H2AX assay based on imaging flow cytometry
Representative images of γ-H2AX foci in human blood lymphocytes irradiated cells with γ-rays (0, 2 and 4 Gy), 1 h after irradiation. Cellular images displayed here show BF, γ-H2AX, γ-H2AX foci mask, DRAQ5 nuclear staining and a composite of γ-H2AX and DRAQ5. The spot counting wizard in the IDEAS® software was used to identify and enumerate γ-H2AX foci in all imagery (40x magnification)