Fig. 1
From: Development of a high-throughput γ-H2AX assay based on imaging flow cytometry
Development of a simple and fast γ-H2AX assay protocol. Fresh blood samples (100 μL) were prepared and cultured in RPMI medium following gamma irradiation. At specific time points up to 24 h after irradiation, whole blood samples were lysed, fixed and stained with γ-H2AX antibody and the nuclei were counter-stained with DRAQ5. Cellular imagery was automatically captured using the ISX INSPIRE™ software that controls the ImageStream®X (ISX) Mark II imaging flow cytometer. All acquired imagery was analyzed by IDEAS® software