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Fig. 8 | Radiation Oncology

Fig. 8

From: Development and characterisation of acquired radioresistant breast cancer cell lines

Fig. 8

Activation of PI3K and MAPK pathways in ER+ derived radioresistant cell lines. a and c MAPK pathway activity; b and d PI3K pathway activity. a Western blot analysis showing the levels of p-ERK in response to a single fractionated dose of 2 Gy radiation in MCF-7, ZR-751 and MDA-MB-231 cell lines in comparison to their RR derivatives. b Western blot analysis showing the levels of p-AKT in response to a single fractionated dose of 2 Gy radiation in MCF-7, ZR-751 and MDA-MB-231 cell lines in comparison to their RR derivatives (2-way ANOVA with Holm-Sidak’s multiple comparisons test; data expressed as mean ± SEM, n = 3, ****p ≤ 0.0001; *p ≤ 0.05). c Heatmap showing log2 mean-centered gene expression profiles between parental and RR cell lines in respect to a MAPK pathway activity gene signature [27]. d Heatmap showing log2 mean-centered gene expression profiles between parental and RR cell lines in respect of the PI3K pathway (associated genes taken from the PI3K KEGG pathway (upper heatmap) and FOXO-regulated genes (lower heatmap) [28]. Heatmap clustering was carried out using Pearson correlation with average linkage

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