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Fig. 5 | Radiation Oncology

Fig. 5

From: Development and characterisation of acquired radioresistant breast cancer cell lines

Fig. 5

WNT signalling is increased in ER+ derived radioresistant cell lines. a Heatmap showing log2 mean-centered gene expression profiles between parental and RR cell lines in respect of the WNT signalling pathway (left heatmap) with selected WNT and FRIZZLED genes shown (center) and WNT target genes (right), taken from the KEGG pathway database [25]; red = higher expression, green = lower expression. The gene list and the order in which they appear in the heatmap are shown in Additional file 6: Table S3. Heatmap clustering was carried out using Pearson correlation with average linkage. b IHC of WNT5a expression using MCF-7 and ZR-751 parental and RR MTS. c Western blot analysis showing the levels of WNT5a in untreated MCF-7and ZR-751 cell lines in comparison to their RR derivatives (samples were obtained at 0 and 24 h after a 2 h serum starve), (unpaired, two tailed t-test; data expressed as mean ± SEM, n = 3, ****p ≤ 0.0001; ***p ≤ 0.001, ** p ≤ 0.01)

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