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Fig. 2 | Radiation Oncology

Fig. 2

From: Transportable system enabling multiple irradiation studies under simultaneous hypoxia in vitro

Fig. 2

Hypoxic conditions are maintained in Minihypoxy chambers. a Hypoxia-inducible factor HIF-1α is equally induced in Minihypoxy system (MH) and in hypoxia workstation (HW) in 1% O2 (HOX). The hypoxic response is shown with two carcinoma cell lines (HeLa, UT-SCC74). b The Minihypoxy chambers (MH) were disconnected from the gas flow (no flow) and the level of HIF-1α was followed for 30 min. The control cells were cultured in hypoxia workstation (HW) for 24 h and then transferred to normal cell incubator with 21% O2 (REOX). The samples were collected accordingly

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