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Fig. 3 | Radiation Oncology

Fig. 3

From: Half brain irradiation in a murine model of breast cancer brain metastasis: magnetic resonance imaging and histological assessments of dose-response

Fig. 3

Flow chart of the processes involved in the quantification of γ-H2AX intensity, tumor nucleus size and tumor cell density. DAPI and γ-H2AX images were overlaid and nuclei were segmented based on DAPI. The intensity of γ-H2AX from segmented nuclei was acquired. From the segmented DAPI images, number and total area of segmented nuclei were quantified. For tumor cell density analysis, tumor clusters were segmented based on DAPI and the area of the cluster was computed

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Radiation Oncology

ISSN: 1748-717X

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