Fig. 3

TTFields Treatment after RT causes the Retention of γH2AX Foci formation. a. U-118 MG cells were irradiated with 4 Gy RT and immediately treated with TTFields for 1 h, 2 h, or 24 h. a pATM (pS1981) or total ATM expression was examined by immunoblotting with α-tubulin used to confirm equal loading. b-c Effect of RT (4 Gy), TTFields or their combination on formation and resolution of γH2AX foci was analyzed by immunofluorescence with DAPI used for counterstaining of cell nuclei. Scale bar - 5 μm. In (c) the average γH2AX foci in cells with more than 5 foci were quantified