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Fig. 1 | Radiation Oncology

Fig. 1

From: Quantitative assessment of radiation dose and fractionation effects on normal tissue by utilizing a novel lung fibrosis index model

Fig. 1

Overview of the experimental setup. a Simultaneous whole thoracic irradiation with 6 MV Linac (red arrows, anterior-posterior direction of horizontal beam) of up to 10 mice fixed with stretched thoraces in vertical position on a custom holder. Radiotherapy (RT) was followed by longitudinal CT imaging every 4 weeks over an overall period of 24 weeks (Endpoint). b CT-scan of animals under inhalation anesthesia. Note the thoracic area with gray hairs, lack of melanin, indicating the irradiated region. c Processing of CT-images; the lung area was semi-automatically segmented (red line) and manually curated slice by slice. Total lung volume (mm3) and mean lung density (HU) provided the basis for calculation of the lung fibrosis index (FI) as indicated in the methods section. (FI = fibrosis index, HU = Hounsfield unit, RT = radiotherapy, fx = fractionation)

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