Fig. 3

Analysis of cytotoxicity, cell cycle effects and DNA double strand break repair of Idelalisib. Primary skin fibroblasts were treated for 48 h with a concentration of 10 nmol/l of Idelalisib. Apoptosis and necrosis were detected by Annexin V-APC/7AAD staining and flow cytometry. a An example of the gating in the FACS plots is shown for untreated, 2 Gy IR, 10 nmol/l and combined treated cells. Induction of b apoptosis and c necrosis at different Idelalisib concentrations with and w/o irradiation of 2 Gy. Cell cycle phase of primary skin fibroblasts was detected by Hoechst 33342 staining. d Induction of G2/M-phase at different Idelalisib concentrations with and w/o irradiation of 2 Gy. DNA double strand breaks were detected by γH2AX immunostaining in primary skin fibroblasts. e Initial DNA double strand breaks after 0.5 Gy and 30 min recovery time and f after 2 Gy and 24 h recovery time. g An example γH2AX, PML-nuclear bodies and nuclear (Dapi) stained cells is given. Significant differences with p = 0.05 are marked as *