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Fig. 4 | Radiation Oncology

Fig. 4

From: PARP-1 depletion in combination with carbon ion exposure significantly reduces MMPs activity and overall increases TIMPs expression in cultured HeLa cells

Fig. 4

Studies of TIMP-1, TIMP-2 and TIMP-3 protein expression by IF after carbon ion exposure (0- 4 Gy). a, c & e Immunostained cells using anti-TIMP-1, anti-TIMP-2 and anti-TIMP-3 antibody followed by FITC conjugated host specific secondary antibody, are shown here respectively. DAPI was used to stain nucleus. Scale bar denotes 20 μm. b, d & f Intensities of the immunostained cells for TIMP-1, TIMP-2 and TIMP-3 respectively were measured by ImageJ software, normalized with beta actin. Normalized expression of respective protein in H-vector without irradiation was considered as 100 % expression of that protein. Accordingly rest were calculated and plotted. Each bar diagram represents the mean ± SD of three independent experiments. ‘**’ & ‘***’ denote 0.01 < p ≤ 0.05 & p ≤ 0.001 respectively for TIMPs expression at each of the particular dose compared with un-irradiated cell-types. ‘#’ & ‘##’ denote 0.01 < p ≤ 0.05 & 0.001 < p ≤ 0.01 respectively for difference of TIMPs expression between H-vector and HsiI at a particular dose

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