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Fig. 2 | Radiation Oncology

Fig. 2

From: PARP-1 depletion in combination with carbon ion exposure significantly reduces MMPs activity and overall increases TIMPs expression in cultured HeLa cells

Fig. 2

Activity of MMP-2 and MMP-9 by gelatin zymography after carbon ion exposure and gamma (0- 4 Gy) irradiation. a Typical photograph of gelatin zymogram after 19 h of post-carbon ion exposure. The clear bands of MMP-9 (92 KDa) and MMP-2 (72 KDa) signify their gelatinolytic activity after irradiation. ‘M’ marked lanes represent molecular weight marker. b & c The intensities of bands were measured by ImageJ software, normalized with loading control (given as Additional file 1) and plotted. The intensities of the representative band of both MMP-2 & MMP-9 in un-irradiated H-vector were considered as 100 % activity and accordingly the activity of the respective MMPs at different doses were calculated and plotted. d, e & f MMP-2 and MMP-9 activities are shown in H-vector and HsiI after gamma irradiation and representative bar diagram was prepared following the procedure as mentioned above. ‘***’ on respective bar denotes p ≤ 0.001 for activity at that dose compared with un-irradiated respective cell-type H-vector or HsiI. ‘###’ denotes p ≤ 0.001 for difference of MMPs activities between H-vector and HsiI at a particular dose

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