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Fig. 7 | Radiation Oncology

Fig. 7

From: The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis

Fig. 7

cIAP1 and XIAP knockdown radiosensitizes colorectal cancer cells. Single cells from knockdown cell cultures were plated in a 3D extracellular matrix and irradiated with 0, 2, 4 or 6 Gy (single doses) 24 h thereafter. Colonies with more than 50 cells were counted microscopically 6–7 days after plating. a Typical 3D colony formation of non-irradiated (0 Gy) or irradiated (2 Gy) mock-treated (mock) or cIAP1/XIAP depleted (cIAP1+X si) cell cultures 6–7 days after plating (bar, 200 μm). b Plating efficiency of 3D grown mock, control siRNA (co), cIAP1 siRNA (cIAP1), XIAP siRNA (X) and cIAP1/XIAP (cIAP1+X) siRNA transfected cells was determined and normalized to control siRNA (co) treated cells. (means ± SD; n ≥3). c For measurement of 3D radiation survival, indicated knockdown cell cultures were irradiated (0–6 Gy, single dose) and survival fractions relative to non-irradiated controls were calculated. Curves were fitted according to the linear quadratic model (means ± SD; n ≥3; *P <0.05; **P <0.01; Student’s t-test in comparison to control (co) siRNA treatment)

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