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Fig. 6 | Radiation Oncology

Fig. 6

From: The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis

Fig. 6

cIAP1 and XIAP depletion hampers DNA double-strand break repair. SW480, and HCT-15 colorectal cancer cells were subjected to siRNA-mediated cIAP1 and/or XIAP knockdown 48 h before irradiation. a Western Blot analysis confirmed knockdown of cIAP1 and XIAP (X) at 48 h after siRNA transfection, while β-actin served as loading control. Immunofluorescence staining for γH2AX (b) or 53BP1 (c) was accomplished 24 h after irradiation with 2 Gy and nuclear foci were counted subsequently (means ± SD; n = 3; * P <0.05; **P <0.01; Student’s t-test). Representative photographs of indicated conditions show staining of nuclei in blue (DAPI), γH2AX in red and 53BP1 in green (scale bar, 5 μm)

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Radiation Oncology

ISSN: 1748-717X

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