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Fig. 4 | Radiation Oncology

Fig. 4

From: The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis

Fig. 4

BV6 treatment decreases basal clonogenic survival and substantially radiosensitizes colorectal cancer cells. Single cells were plated in a 3D extracellular matrix and 24 h thereafter, cells were treated either with DMSO or increasing concentrations of BV6 (0.1, 0.25, 1, 1.5 μM) or were left untreated (mock). At 4 h after BV6 treatment, cells were irradiated with 0, 2, 4 or 6 Gy (single doses) and ≥6 days after plating colonies >50 cells were microscopically counted. a Typical colony formation of DMSO- or BV6-treated colorectal cancer cell lines ≥6 days after plating (bar, 200 μm). b Survival curves of indicated colorectal cancer cell lines after BV6 treatment were calculated and normalized to DMSO-treated cells (means ± SD; n ≥3; *P <0.05; **P <0.01; Student’s t-test). c To evaluate 3D radiation survival, single cells were pretreated for 4 h with DMSO or increasing concentrations of BV6 or left untreated (mock). Subsequently, cells were irradiated (0–6 Gy, single dose) and survival fractions relative to non-irradiated controls were calculated. Curves were fitted according to the linear quadratic model (means ± SD; n ≥3; *P <0.05; **P <0.01; Student’s t-test in comparison to DMSO-treated controls)

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