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Fig. 8 | Radiation Oncology

Fig. 8

From: The in vitro immunogenic potential of caspase-3 proficient breast cancer cells with basal low immunogenicity is increased by hypofractionated irradiation

Fig. 8

Surface expression of activation markers by dendritic cells after contact with supernatants of MCF-7 or MDA-MB231 breast cancer cells. The surface expression of CD80, CD86, and DC-SIGN on DCs was analyzed by flow cytometry (representative primary data are displayed in a1 and b1) after incubation for two days of immature DCs with cell culture supernatants of MCF-7 (a) or MDA-MB231 (b) tumor cells treated with hypofractionated irradiation (4x4Gy or 6x3Gy) and/or hyperthermia (41,5 °C for 1 h). Ionizing irradiation was applied 4 h before or after hyperthermia on the first and last day of fractionation. Representative data of one out of three experiments, each performed in triplicates, are presented as mean ± SD in a2 for MCF-7 cells and b2 for MDA-MB231 cells. DCs: dendritic cells; Gy: Gray; HT: hyperthermia; MFI: mean fluorescence intensity; w/o: mock treated control; neg: immature DCs in medium only; pos: immature DCs incubated with IL-1β, PGE2, IL-6 and TNFα instead of tumor cell supernatants; *p <0,05, **p <0,01 compared to w/o

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Radiation Oncology

ISSN: 1748-717X

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