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Fig. 4 | Radiation Oncology

Fig. 4

From: The in vitro immunogenic potential of caspase-3 proficient breast cancer cells with basal low immunogenicity is increased by hypofractionated irradiation

Fig. 4

Forms of MCF-7 and MDA-MB231 breast cancer cell death after hypofractionated irradiation and/or hyperthermia. MCF-7 (a, c) or MDA-MB231 (b, d) breast cancer cells were treated with hypofractionated irradiation (4x4Gy or 6x3Gy) and/or hyperthermia (41,5 °C for 1 h). Ionizing irradiation was applied 4 h before or after hyperthermia on the first and last day of fractionation. 24 h after the last treatment, the cells were harvested. The cells were then stained with AnxA5-FITC/PI and cell death was analyzed by flow cytometry. The percentages of apoptotic (AnxA5+/PI-) and necrotic (AnxA5+/PI+ and PI++) tumor cells are displayed in (a and b). For determination of the DNA content, the cells were stained with PI in the presence of detergent and also analyzed by flow cytometry. The percentage of cells with subG1 DNA-content is displayed in (c and d). Representative data of one out of three experiments, each performed in triplicates, are presented as mean ± SD. Gy: Gray; HT: hyperthermia; w/o: mock treated control. *p <0,05, **p <0,01 compared to w/o; #p <0,05, ##p <0,01 related to only irradiated samples

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Radiation Oncology

ISSN: 1748-717X

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