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Fig. 2 | Radiation Oncology

Fig. 2

From: The in vitro immunogenic potential of caspase-3 proficient breast cancer cells with basal low immunogenicity is increased by hypofractionated irradiation

Fig. 2

Forms of MCF-7 breast cancer cell death after irradiation and/or hyperthermia. MCF-7 breast cancer cells were irradiated with single doses of ionizing radiation and/or treated with hyperthermia (41,5 °C for 1 h). The cells were irradiated 4 h after HT treatment. 24 h (a) or 72 h (b) after the last treatment, the cells were stained with AnxA5-FITC/PI and cell death was analyzed by flow cytometry. The percentages of apoptotic (AnxA5+/PI-) and necrotic (AnxA5+/PI+ and PI++) tumor cells are displayed. Representative data of one out of two experiments, each performed in triplicates, are presented as mean ± SD. Gy: Gray; HT: hyperthermia; w/o: mock treated control. *p <0,05, **p <0,01 related to w/o

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