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Fig. 3 | Radiation Oncology

Fig. 3

From: The Focinator - a new open-source tool for high-throughput foci evaluation of DNA damage

Fig. 3

The Focinator counts foci for each pre-selected ROI automatically. Figure 3 demonstrates the calculation process stopped at the automated foci finding step for all ROIs. The image shows the selected foci in ROI 4. This part of the automation is based on the user’s noise level settings and on the previously marked ROIs, which are directly imported to the foci channel. Foci counting is followed by the closing of all channels and the immediate export into data files. The ROIs information will be imported into the export files in the order they were displayed in the ROI Manager window and named as numbers starting from one

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