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Figure 5 | Radiation Oncology

Figure 5

From: A cell-based high-throughput screening assay for radiation susceptibility using automated cell counting

Figure 5

Detection of radiosensitization by automated cell counting. (a) PC-3.pLKO.1-EV cells (open circles) and PC-3.pLKO.1-shPRKDC cells (closed circles) were subjected to 0–6 Gy IR and dose-responses were determined by CFA. (b) PC-3.pLKO.1-EV cells (open circles) and PC-3.pLKO.1-shPRKDC cells (closed circles) were seeded 1,000 cells/well, subjected to 0–6 Gy IR and dose-responses were determined by automated cell counting. (c) PC-3 cells seeded 1,000 cells/well and cultured with (closed circles) or without (open circles) caffeine were subjected to 0–6 Gy IR and dose-responses were determined by automated cell counting. Graphs were drawn after fitting the data to the linear quadratic equation. Data shown are means with SEM of three independent experiments. The α and β values of the equations are given in Table 2.

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