Figure 4

The effect of ART and radiation on HeLa (A) and SiHa (B) cell cycle progression. Cells were treated with or without 2.0 μmol/L (HeLa) or 2.5 μmol/L ART (SiHa) for 24 h prior to exposure to 6 Gy irradiation (IR). After 24 h, both attached and floating cells were harvested for cell cycle analysis. The expression of Wee1, Cyclin B1 and Cdc2 in HeLa (C) and SiHa (D) cells. HeLa and SiHa cells were treated with or without ART for 24 h prior to exposure to 6 Gy of X-ray irradiation (IR). The expression levels of Wee1, Cyclin B1, Cdc2 and the internal control β-Actin were evaluated by Western blot.