Figure 3From: Establishment of a small animal tumour model for in vivo studies with low energy laser accelerated particlesCharacterization of the tumour micromilieu at starting size. Representative pictures of KHT mouse sarcoma characterized at start of treatment with a size of 2 mm in diameter: (a) The limited and bordered tumour volume was confirmed by standard H&E staining, showing (b + c) the integrity of cellular components by differentiating the basophil stained nuclei from the eosinophil stained cytoplasm, whereas necrosis of single cells cannot be excluded (Figures b and c are higher magnifications of Figure a). (d) The analysis of the tumour histology revealed a well perfused tumour (blue, Hoechst 33342) that is interspersed with small vessels (red, CD31) and some hypoxic area (green, pimonidazole).Back to article page