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Table 3 Double immunofluorescence combinations

From: The roles of platelet-derived growth factors and their receptors in brain radiation necrosis

Primary Dilution Secondary Primary Dilution Secondary
PDGF-C 1:50 F488 CD68 1:25 F546
PDGF-C 1:50 F488 hGLUT5 1:50 F546
PDGF-C 1:50 F488 GFAP 1:25 F546
PDGF-C 1:50 F488 CD45 1:50 F546
PDGF-D 1:20 F488 CD68 1:25 F546
PDGF-D 1:20 F488 hGLUT5 1:50 F546
PDGF-D 1:20 F488 GFAP 1:25 F546
PDGF-D 1:20 F488 CD45 1:50 F546
PDGFR-α 1:10 F488 CD68 1:25 F546
PDGFR-α 1:10 F488 hGLUT5 1:50 F546
PDGFR-α 1:10 F488 GFAP 1:25 F546
PDGFR-α 1:10 F488 CD31 1:20 F546
PDGFR-β 1:20 F488 CD68 1:25 F546
PDGFR-β 1:20 F488 hGLUT5 1:50 F546
PDGFR-β 1:20 F488 GFAP 1:25 F546
PDGFR-β 1:20 F488 CD31 1:20 F546
  1. Primary, primary antibody; Secondary, secondary antibody; F488, Alexa Fluor 488; F546, Alexa Fluor 546.