Double immunofluorescence staining. Double immunofluorescence staining from case 1 revealed that PDGFR-α and β were strongly expressed in CD31-positive cells in PN (D and I). PDGFR-α positive cells were merged with many cells positive for CD68 (A), GFAP (B), hGLUT5 (C), and CD45 (E). PDGFR-β-positive cells merged specifically with endothelial cells (F, G, H, I and J, *). Endothelial cells (*) were nonspecifically stained with secondary fluorescence antibody. The scale bar represents 50 μm.