Double immunofluorescence staining. The results of double immunofluorescence staining from case 1 revealed that PDGF-C or D-positive cells were merged with many CD68 (A, E), GFAP (B, F), hGLUT5 (C, G), and CD45 (D, H) -positive cells in PN. Some PDGF-C or D-positive cells did not express CD68, GFAP, hGLUT5 or CD45 and vice versa. Endothelial cells (*) were nonspecifically stained with secondary fluorescence antibody. The scale bar represents 50 μm.