Induction of surface NKG2D ligands by TSA treatment was blocked by CHX in A549 cells. A549 cells were treated with CHX (1 μg/ml) and 250 nM TSA for 18 hours, and single treatment of CHX reduced the transcription of NKG2D ligands (A). However, the transcriptional induction of NKG2D ligands by TSA was not blocked by co-treatment with CHX (B). Although the suppressions of ULBP1-3 were not statistically significant, CHX effectively blocked the surface expression of MICA and MICB by TSA. Filled gray, filled dark gray, thick line and thin line represent isotype control, untreated control, CHX or TSA single treatments, and TSA plus CHX combined treatment, respectively. Significant differences between untreated control and treated cells are presented as * (p < 0.05) or ** (p < 0.01).