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Figure 3 | Radiation Oncology

Figure 3

From: Synergistic enhancement of NK cell-mediated cytotoxicity by combination of histone deacetylase inhibitor and ionizing radiation

Figure 3

Increased susceptibility of A549 cells to the cytolytic activity of NK-92 cells following HDAC inhibitor and ionizing radiation co-treatment. A549 cells were not treated (open circle), irradiated with 8 Gy (filled circle), treated with 125 ng/ml apicidin or 250 nM TSA (filled square), or co-treated with after ionizing radiation plus HDAC inhibitors (apicidin or TSA; filled triangle). Cells were co-cultured with NK-92 cells at the indicated effector/target ratio. To determine the specificity of NKG2D-mediated cytolysis, NK-92 cells were pre-incubated with blocking mAb against NKG2D (open triangle). All experiments were performed in triplicate and significant differences between NK cell-mediated lyses of untreated and treated cells were accepted for P values of < 0.05.(*; p < 0.05, **; p < 0.01).

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