Analysis of the expressions of NKG2D ligands after treating lung cancer cells with HDAC inhibitors or ionizing radiation. Multiplex RT-PCR analysis was performed to determine the mRNA expressions of NKG2D ligands. A549 and NCI-H23 cells were treated with 125 ng/ml apicidin, 2.5 μ M SAHA, or 250 nM TSA (A, B) for 12 hours. A549 cells were irradiated with the indicated doses and allowed to recover for 24 hours (C). Amplified DNA was quantitated using Quantity-one software (Bio-rad). All experiments were performed at least three times, and representative results are shown. MICA/B: MHC class I chain-related molecules A/B, ULBP1/2/3: UL16-binding proteins 1/2/3, ACTB: -actin, RPL19: ribosomal protein19.