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Figure 2 | Radiation Oncology

Figure 2

From: RAD9 deficiency enhances radiation induced bystander DNA damage and transcriptomal response

Figure 2

Effect of RAD9 status on micronuclei formation in H1299 cells A. Western blot of RAD9 protein in H1299 cells. Lane 1, untransfected H1299 cells, lanes 2 and 3, two stable clones transfected with shRAD9. Western blot analysis was performed using RAD9 and β-actin antibodies. Image J was used to determine the level of RAD9 protein after knockdown and the abundance of RAD9 indicates expression normalized to untransfected control H1299. B. IR-Induced Micronuclei (MN): micronucleus formation in H1299 (grey bars) and H1299shRAD9 (striped bars) cells after direct (IR) or bystander (BYS) exposure to 1 Gy of α particles. Data were pooled from five independent sets of experiments each having n ≈ 35 and results represent mean ± SD. Single asterisk indicates a significance of p < 0.05 and triple asterisk, a p < 0.001. C. MN fold induction values plotted as a function of number of dishes with each fold change for directly irradiated cells (IR). H1299 (grey line) and H1299shRAD9 (dashed black line). D. same as C, except bystander cells (BYS) were assessed.

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