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Table 1 Genes upregulated upon radiation

From: mRNA-based vaccines synergize with radiation therapy to eradicate established tumors

Pathway/function

Genes upregulated upon tumor radiation

T cell signaling

Cd8b1 (2.8), Cd8a (2.6), Il12r (2.2), Il2r (2.1), Gzmb (3.8), Gzmk (1.9), Prf1 (2.2), Icos (2.4), Ctla4 (1.4), Eomes (1.6), Stat1 (1.6), Stat4 (2.4)

NK cell signaling

Klrd1 (3.1), Klrk1 (2.1), Klrc1 (3.0), Klra2 (1.6), Nkg7 (4.0), Il12r (2.2), Gzmb (3.8), Gzmk (1.9), Prf1 (2.2), Fcgr4 (2.2)

Chemotaxis

Ccl3 (2.0), Ccl5 (2.5), Ccl8 (2.1), Ccl11 (2.2), Cxcl12 (1.6), Cxcl16 (2.1), Ccrl2 (2.1), Ccr5 (1.7), Cxcr3 (1.5)

TLR signaling

Tlr7 (1.9), Tlr8 (2.2), Tlr9 (1.6), Tlr13 (1.7), Irak3 (1.5), Aebp1 (2.5)

MHC class II presentation

H2-Dma (1.8), H2-Ab1 (1.7), H2-Eb1 (1.5), H2-Aa (1.9), H2-T24 (1.8), Cd74 (1.7), Cathepsins: k, s, c, h, w, a (1.6-2.0), Anpep (1.9)

Complement system

C1qa (2.0), C1qb (1.6), C1qc (2.1), C1r (1.7), C2 (2.2), C3 (2.0), C4b (2.6), Cfb (2.1), Cfh (1.9)

Dendritic cells, macrophages

Cd11c (Itgax) (1.9), Cd11b (Itgam) (1.7), Clec4a1 (2.1), Clec4a3 (2.2), Ly6i (2.3), Sema4 (1.9), Blnk (1.6), Lrp1 (2.6), Aebp1 (2.5), Nos2 (2.4)

Adhesion

Vcam1 (1.8), Lamc1 (1.8), Tns1 (1.8), Cd38 (1.8), Itgam (1.7), Itgax (1.9), Thbs1 (1.5), Thbs2 (2.2), Clec4a1 (2.1), Clec4a3 (2.2), Antxr (2.6)

Angiogenesis

Ecm1 (2.1), Ptafr (1.6), Hpse (2.1), Atxr1 (2.6), Fgfr1 (1.6), Mmp13 (4.1), Mmp19 (1.7), Thbs1 (1.5), Nrp2 (1.8), Nrp1 (2.4), Crim1 (1.5), Ace (2.1), Plxdc2 (1.8)

  1. C57BL/6 mice (4 mice per group) were challenged s.c. on the right limb with 3 × 105 syngenic E.G7-OVA tumor cells. Mice were treated starting at day 13 with local radiation of 6 Gy applied in 3 equal doses on 3 consecutive days. Untreated mice served as controls. After 7 days tumors were exercised, RNA was isolated and gene expression was analyzed. Differentially expressed genes were identified by statistical analysis with the p ≤ 0.05 and a fold change of at least 1.5. The exact fold change is presented in the brackets.