Figure 3
From: Down-regulation of BTG1 by miR-454-3p enhances cellular radiosensitivity in renal carcinoma cells
Down-regulation of BTG1 significantly increases cellular radiation sensitivity in 786-O cells. (A) The down-regulation of endogenous BTG1 protein following transfection with siRNA oligonucleotides against BTG1 (siRNA-BTG1) or nonsense small RNA oligonucleotides (negative control, NC) were compared to untransfected (blank) cells. GAPDH serves as loading control. Fold changes of protein levels represent the average of three separate blots. (B) The percentage of 786-O cell viability after transfection with siRNA oligonucleotides against BTG1 (siRNA-BTG1) or nonsense small RNA oligonucleotides (negative control, NC) was determined by trypan blue dye exclusion assay at 48 h after X-ray exposure. (C) Effects of nonsense small RNA oligonucleotides or siRNA-BTG1 over-expression on caspase-3 activity in 786-O cells without irradiation or with 5 Gy of X-rays. (D) Colony formation assay of 786-O cells transfected with NC or siRNA-BTG1. (E) Representative plates of survival fraction of 786-O cells after various treatments. The data (means ± SE) are representative for at least five independent experiments.