Figure 1
From: Down-regulation of BTG1 by miR-454-3p enhances cellular radiosensitivity in renal carcinoma cells
BTG1 is induced in response to various extracellular stressors in 786-O cells. (A) Western blot assay of BTG1 at indicated time points after treatment of human renal carcinoma 786-O cells with 5 Gy of X-rays, DMSO (1.6%, v/v), and serum deprivation. GAPDH is used as a loading control. Fold changes of protein levels represent the average of three separate blots. (B-D) BTG1 mRNA levels were monitored by qRT-PCR at 0, 4, and 8 h after treatment of 786-O cells with (B) 5 Gy of X-rays, (C) DMSO (1.6%, v/v) and (D) serum starvation. Values were normalized to GAPDH. Results represent means ± SE of four independent experiments.