sHsp70 serum levels in blood of patients with SCCHN. A. sHsp70 concentrations in sera of patients (n = 21) before therapy compared to healthy donors (n = 28). B. sHsp70 serum data from patients were separated into two groups (high n = 11, low n = 10) reflecting the mHsp70 membrane expression as measured in A. C. Standard box plots are shown with boundaries indicating the 25th and the 75th percentile. The line inside boxes indicates the median and the whiskers indicate the 10th and 90th percentile, respectively. All outliers are shown. (***p < 0.001) C. ROC curve of data from (A) were attained by SigmaPlot software showing an AUC of 0.9101 (p < 0.0001, CI 95%). The cut-off level was 2.5 ng/ml with a sensitivity of 89% and a specificity of 91%). Abbr.: ROC, receiver operating characteristic; AUC, area under the curve; CI, confidence interval.