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Figure 4 | Radiation Oncology

Figure 4

From: Increased radiosensitivity and radiothermosensitivity of human pancreatic MIAPaCa-2 and U251 glioblastoma cell lines treated with the novel Hsp90 inhibitorNVP-HSP990

Figure 4

The effects of treatment with NVP-HSP990, hyperthermia and 4 GyIR on cell proliferation and cell death of U251 (A, B, C) and MIAPaCa-2 cells (D, E, F). In figure A and D blackbars represent non-irradiated cells while grey bars represent cellswhich were irradiated with a single dose of 4 Gy. The cells weretreated with 0.05 μM NVP-HSP990 and 42°C for1 hour and then replaced to 37°C for the next 23 hoursin the presence of the inhibitor. Thereafter the culture medium wasreplaced with fresh medium without inhibitor and the cells wereirradiated with a single dose of 4 Gy. 72 hours later, thecells were stained with trypan blue to determine the number of viablecells (A, D). Mean value and SEM from 3 independentexperiments is shown. To detect the percentage of apoptotic/necroticcells, cells were stained with FITC-conjugated anti-Annexin V antibodyand propidium iodide (B, C, E, F). Blackbars represent apoptotic while grey bars necrotic cells. Mean values andrange of 2 independent experiments are shown.

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