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Figure 3 | Radiation Oncology

Figure 3

From: Increased radiosensitivity and radiothermosensitivity of human pancreatic MIAPaCa-2 and U251 glioblastoma cell lines treated with the novel Hsp90 inhibitorNVP-HSP990

Figure 3

Influence of NVP-HSP990 and hyperthermia on the radiosensitivity ofU251 and MIA PaCa-2 cells (A and B). The cells were incubatedwith 0.02 or 0.1 μM NVP-HSP990 for 24 h. Thereafter,growth media containing NVP-HSP990 were replaced with fresh medium andthe cells were irradiated with single IR doses ranging between 2 and8 Gy (C and D). In the case of the combinedtreatment with NVP-HSP990 and hyperthermia, U251 cells (C) or MIAPaCa-2 cells (D) were simultaneously treated with0.05 μM NVP-HSP990 and 42°C for 1 hour, thenreplaced to 37°C for the next 23 hours and then irradiated asdescribed above. Colony forming efficiency was determined 12 dayslater and colonies containing at least 50 cells were scored. Data showmean values and range of 2 independent experiments each plated intriplicate.

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Radiation Oncology

ISSN: 1748-717X

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