Figure 3
From: Regulation of protein translation initiation in response to ionizing radiation
Inhibition of protein translation results in rapid Mcl-1 decline. Non-irradiated or, alternatively, 24 h after IR with 10 Gy, Jurkat cells were treated with 1 μM (CHX) for 0–120 min. Cells were lysed at respective time points. Mcl-1 levels were analyzed by Western blot (upper panel). Mcl-1 levels were quantified by densitometry and normalized to the Mcl-1 level of untreated non-irradiated control cells. The values were fitted and the half-life of Mcl-1 was calculated (lower panel).