Figure 6

Histological staining of cells grown as three-dimensional colonies and monolayers. (a, b) Representative images of sections of CAEP-derived MCTS stained with hematoxylin and eosin showing pathophysiological features of solid tumors (necrosis, apoptosis, dermal junctions and mitosis). Histological staining was performed on sections obtained from tumor spheres that were included in collagen (StemCell Technologies) after 15 days of culture, fixed in 10% buffered formalin and embedded in paraffin. (c, d) Hematoxylin-eosin staining of CAEP cells grown on the Lab-Tek® Chamber Slide™ System (Nalge Nunc International). Before staining, cells were directly fixed on the slide with 10% buffered formalin. Photomicroscope analysis was performed using Axioscope 40 microscope (Zeiss, Milan Italy).