Figure 3
From: In vitro irradiation system for radiobiological experiments
Treatment planning for cell culture irradiation. (a, b) Screenshot showing the source-axis distance (SAD), the dimensions of the parallel opposed fields and the target coverage used for MCTS irradiation. The SAD measured 100 cm, while the source surface distance (SSD) was 96 cm. (c) A CT scan was performed in the set-up phase of the phantom containing the microplate (in which wells were filled with culture medium) in order to optimize dose delivery, exactly as would be done for a patient. Two opposed fields (AP at 0° and PA at 180°) of 20x20 cm2 were chosen for irradiation. The irradiation system, independently of dose, ensured the delivery of a uniform dose to the yellow areas containing the microplate. A Philips Pinnacle3 radiation therapy planning system (Philips Healthcare) was used and customized with the geometric and dosimetric characteristics of an Elekta Synergy linear accelerator.